Genomic Know-How® Webinar Series: Best Practices for Obtaining RNA Sequencing Data from Formalin-fixed Paraffin-embedded (FFPE) Samples

Life Sciences, Pharmaceutical Regulation, Pharmaceutical, Drug Discovery & Development, Drug Safety, Commercialization & HEOR,
  • Friday, April 25, 2014

Formalin-fixed Paraffin embedded (FFPE) tissues present many sample preparation, extraction and method development challenges. Despite these difficulties, interest in genomic analysis of FFPE samples remains high, and large collections of clinically-annotated FFPE material await analysis. The presenter will describe the methods and best practices based on the extensive work Quintiles has performed analyzing both RNA and DNA from FFPE samples.

This webinar is intended to help genomic researchers better understand technical hurdles in the analysis of FFPE-derived RNA to:

  • Learn how EA has overcome challenges inherent in FFPE samples
  • Gain insight into experimental design considerations
  • Learn how to exploit your collection of FFPE material

Viewers will learn:

  • How RNA sequencing data derived from fresh frozen and FFPE material can be compared
  • How to design experiments that obtain meaningful data from your FFPE samples


Patrick Hurban, PhD, Global Head of Genomic Research and Development, Quintiles

Dr. Hurban joined Quintiles in 2012 as Global Head of Genomic Research and Development, upon the acquisition of Expression Analysis by Quintiles. He is responsible for the identification and implementation of new genomic capabilities, for consulting on special projects, and for the development and validation of genomic assays. These include broad-based screening assays such as exome and RNA sequencing, as well as microarray-based expression profiling and genotyping, to highly targeted assays. Dr. Hurban has over 25 years of experience in molecular genetics, including over 15 years in positions of increasing responsibility in high-profile genomics-focused organizations. His research interests have focused on the genetic control of gene expression and have spanned diverse fields such as toxicology, developmental and cancer biology.

Following a Bachelor’s degree in Biology from the University of North Carolina at Chapel Hill, he earned his Ph.D. in Human Genetics from the University of Utah in Salt Lake City before serving as a Postdoctoral Research Fellow of the American Cancer Society in the Department of Developmental Biology at Stanford University.

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Who Should Attend?

Discovery and Development Scientists from large pharmaceutical and small-midsize biotechnology companies

Xtalks Partner

EA | Quintiles

Quintiles provides cutting-edge genomic sequencing, gene expression, genotyping, and bioinformatics services to pharmaceutical companies, diagnostic test developers, government agencies, and academic labs. EA | Quintiles conducts projects under clinical-grade quality control and offers bioinformatics expertise and computational infrastructure to process genomic data with consistency and speed.

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