Discover how the optimized target enrichment workflow from Roche NimbleGen and Kapa Biosystems enables improved performance in targeted resequencing.
In NGS target enrichment, researchers face challenges with low sample availability, sequencing of AT- or GC- skewed regions, or damaged DNA as in FFPE samples. By combining the SeqCap EZ products’ capture efficiency with Kapa’s expertise in library prep and high fidelity amplification, we have an effective solution for you to overcome these challenges.
During this webinar our R&D experts will share data demonstrating how our workflow will help you achieve greater molecular complexity with minimal DNA sample input or with FFPE DNA, hence maximizing variant discovery with your precious samples.
Speakers
Maryke Appel, PhD, Technical Director, Kapa Biosystems
Maryke Appel, PhD, Technical Director, Kapa Biosystems
Dr. Appel received a PhD degree in Biochemistry from the University of Stellenbosch in South Africa in 2001. The focus of her post-graduate research was molecular plant-pathogen interactions. Prior to becoming Kapa’s first employee in 2006, Dr Appel worked for a small biotech developing novel, targeted cancer therapeutics. At Kapa, she initially formed part of the Research & Development team. She participated in establishing Kapa’s technology platform, and was responsible for developing the KAPA2G and KAPA Blood product lines. Since 2008, Dr Appel has played a leading role in Technical Support and Operations at Kapa. She currently leads the technical team in efforts to overcome the challenges of NGS library preparation.
Daniel Burgess, PhD, Group Leader Sequence Capture, Roche NimbleGen
Daniel Burgess, PhD, Group Leader Sequence Capture, Roche NimbleGen
Dr. Burgess holds a PhD degree from the Department of Human Genetics at the University of Michigan. He was a Postdoctoral Fellow and then Assistant Professor of Neurology at Baylor College of Medicine in Houston, Texas, where he published more than a dozen papers on ion channel genetics and function in epilepsy and other important neurologic disorders.
Dan joined Roche NimbleGen in 2008, where he currently leads efforts to develop cutting edge technologies for high resolution genome analysis. He has been a key contributor to the target enrichment product portfolio, including expansion of the SeqCap EZ product line from human exome to custom developed non-human products and collaborator designed gene panels.
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Roche
Roche NimbleGen is an innovator in the life sciences market focused on research activities and manufacturing target enrichment probe pools for DNA sequencing. Capitalizing on the efficiencies inherent with parallel enrichment, researchers can now design economical, high throughput, and time-saving next-generation sequencing experiments. Next-generation sequencing combined with Sequence Capture probe pools offers researchers a clearer understanding of genomic structure and function in order to understand the impact of genes on biological processes. We are part of the innovations in sequencing solutions for life science research, now and in the future.
Kapa Biosystems
Kapa Biosystems is a life science reagents supplier that employs proprietary, directed evolution technologies to optimize enzymes for PCR, real-time PCR, next generation sequencing and molecular diagnostic applications. Kapa Biosystems offers a portfolio of best-in-class products containing novel enzymes that confer significant performance advantages when compared to traditional wild-type enzymes. The company is based in Wilmington, Massachusetts with a research, development and manufacturing facility in Cape Town, South Africa.
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