Fluorescently-Labeled Peptides – Choosing the Best Dye and Designing the Best Peptide

Peptides fulfill numerous important tasks in nature. The investigation of such peptides in their natural environment is complex and requires specific techniques such as confocal microscopy. In order to study peptides with a confocal microscope, the peptide needs to be labeled with a fluorescent dye. A multitude of fluorescent dyes have been developed over the last decades, and are commercially available as starting materials for the synthesis of fluorescent peptides.

In this webinar, the speakers will provide an overview of known dye labels and highlight new developments in this field (e.g. dyes suitable for the study of complex processes, such as receptor-ligand interactions or the visualization of cellular structures). The speakers will also illustrate how to compare the performance of individual dye labels and give an overview over the commercially available options.

During the webinar, you will learn how to design the right substrate for your scientific application and how to attach the correct dye to the peptide sequence. In addition, you’ll get an overview of different synthetic methods as well as common pitfalls in the design of fluorogenic substrates. For more routine analyses, different decision pathways for the choice of substrates and dye labels will be discussed. The speakers will also demonstrate the use of an innovative new dye selector, which allows you to find suitable substrates for your required spectroscopic properties.

Speakers

Dr. Susanne Rodler, Sales Manager Custom Synthesis, Bachem

Susanne Rodler is Sales Manager Custom Synthesis at Bachem AG. Prior to Bachem, Susanne Rodler worked for a big pharmaceutical company, where she was involved in clinical studies for cancer treatment. Susanne Rodler is a chemist by training and received her PhD for her work on the synthesis of organic dyes.

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Dr. Stefanie Dobitz, Sales Manager Custom Synthesis, Bachem

Stefanie Dobitz is Sales Manager Custom Synthesis at Bachem AG. Before joining Bachem, Stefanie Dobitz earned her doctoral degree from ETH Zurich where she worked on the synthesis and biological evaluation of oligoproline-based peptides for tumor targeting. Previously, she studied chemistry at the Justus Liebig University Giessen, receiving her B.Sc. and M.Sc. for her work on stereoselective oxidations using peptide-based organocatalysts.

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Dr. Erik Schrader, Marketing Specialist, Bachem

Erik Schrader is Marketing Specialist at Bachem AG. Before joining Bachem, Erik Schrader earned his doctoral degree in chemistry from ETH Zurich where he worked on the synthesis and application of acylphosphines. Before his doctoral studies, he worked for half a year at a big chemical company on the synthesis of additives for performance materials.

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