Poseida is seeking an outstanding candidate to join our immuno-oncology team. The Research Associate I/II will support the development of chimeric antigen receptor (CAR-T) cellular therapies against cancer, and possibly other disease areas.  Primary responsibilities will involve hands on designing and performing experiments, organizing and sharing data, isolation of primary lymphocytes for pre-clinical research and the coordination of research projects with the assistance of a senior lab member and/or your manager.

Responsibilities 

These may include but are not limited to:

• Support preclinical development of CAR-T therapies
• Studies may include work with molecular biology and cloning techniques, culture of human cell lines, virus production
• Plan and execute flow cytometry-based assays for characterization of cell therapy products and analysis of tissue samples from animal studies
• Cell separation of T cells and other lymphocytes from human blood products using automated cell processing equipment as well as density gradient centrifugation.
• Studies may include generation of genetically modified human T cells for product development and/or for understanding product biology
• Communicate experimental results and issues in a concise and timely manner to colleagues
• Present data (written and oral) in internal and cross-functional meetings, as required

Requirements, Knowledge, Skills and Abilities

• Research Associate: BS or MS in a Scientific discipline with 0-2+ years relevant industry experience (an equivalent combination of education and experience may be considered)
• Research Associate II: BS or MS in a Scientific discipline with 2 - 5 years relevant industry experience (an equivalent combination of education and experience may be considered)
• Expertise in characterization, culture, expansion, and manipulation of T cells
• Strong knowledge of cellular and/or tumor immunology principles, technologies, and experimental techniques (cell culture, in vitro cellular assays, flow cytometry, etc.)
• Proficiency in multi-parameter flow cytometry and FACS data analysis via FlowJo
• Functional assays measuring cellular activation, cytokine release, proliferation, and killing
• Molecular biology, Cloning and PCR technologies, genetic manipulation
• Cell separation technologies (e.g., Miltenyi AutoMACS) and FACS sorting
• Hands-on experience developing, testing, and/or utilizing CARs
• Electroporation techniques and technologies (Plasmid DNA, IVT RNA)
• Demonstrated track record in successfully developing and employing in vitro/in vivo models
• Genetic manipulation using CRISPRs, TALENs, or ZFNs
• Excellent record keeping & data management skills required
• Ability to organize, communicate, and present complex data sets to key stakeholders from diverse backgrounds
• Excellent communicator with track record of working effectively as an individual contributor and in teams

Research Associate I Pay Rate: $28.37/hour to $37.44/ hour.

Research Associate II Pay rate: $35.48/hour to $42.00/hour

The final pay offered to a successful candidate will be dependent on several factors that may include but are not limited to the type and length of experience within the job, type, and length of experience with the industry, education, geographic location, etc.

Who We Are and What We Do 

Based in San Diego, Poseida Therapeutics is a clinical-stage biopharmaceutical company utilizing our unique and proprietary genetic engineering platform technologies to create next-generation cell and gene therapies with the capacity to cure. We are passionate about making an impact on patients’ lives with the development of our CAR-T therapies in various cancers and gene therapies for rare diseases. Our goal is to deliver potential single treatment cures for patients in need. Our portfolio of product candidates is specifically designed to overcome the limitations of current generation cell and gene therapeutics.