Mass-directed Fraction Collection in High Performance Liquid Chromatography

High-performance liquid chromatography formerly high pressure liquid chromatography (HPLC) has been a staple in laboratory settings for decades for mixture separation and compound purification. Modular in design, an important part of the HPLC workflow is the use of an appropriate detector to monitor chemical compounds eluting off the HPLC system. Monitoring ultraviolet (UV) wavelength absorbance has been the traditional high performance liquid chromatography standard for detection due to its low cost and flexibility.

Typical UV detectors in today’s market can monitor a wide range of wavelengths with high sensitivity. UV detection can also provide powerful quantitative rather than qualitative analysis with chemical standards. However, with its many advantages, there are still many shortcomings when using UV detection that cannot be circumvented without using other more expensive detection methods.

In this webinar, attendees will learn the basics of mass spectrometry and common mass spectrometry ionization techniques — electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI). They will also learn how mass spectrometry can increase efficiency and flexibility in developing chromatography workflows by allowing targeted purification and collection.

Join this webinar to gain insights on how mass spectrometry can supplement other detection methods such as UV detection in addition to being a viable alternative detection method.

With over 50 years of experience, Gilson has the knowledge, expertise and equipment to support the scientific community in developing and expanding chromatography capabilities in both a robust and affordable way for laboratories large and small.

Speakers

Melanie Cathman, Test and Evaluation Manager, Gilson

Melanie Cathman is the Test and Evaluation Manager at Gilson, Inc., and has over 24 years of experience at Gilson in the development, evaluation, validation and support of lab automation and chromatography instruments. She has received BA degrees in Biology and Environmental Studies from Cornell College in Mt. Vernon, Iowa. She is committed to ensuring Gilson products allow scientists to produce verifiable science by confirming the quality of new and existing products.

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Jordan Ho, PhD, Applications Specialist, Gilson

Jordan Ho, PhD is an Applications Specialist for Gilson’s automated liquid handling line. He received his bachelor’s in chemical biology from the University of California-Berkeley and his doctorate from the Massachusetts Institute of Technology. He brings almost a decade of research experience in organic chemistry, biochemistry and chemical biology to support scientists in achieving their automation goals, efficient workflows and verifiable results.

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