Enriching High Titer IgG-Producing Clones Using Cold-Capture and The Namocell Pala Single Cell Dispenser

Cloning of Chinese hamster ovary (CHO) cells expressing recombinant antibodies is a crucial step in the development of monoclonal antibody (mAb) therapeutics. It is important to identify clones that produce high yield. However, the heterogeneity of CHO cells can give rise to varied IgG production among individual cells. Thus, the process of finding high titer clones typically involves screening of large numbers of clones, making it laborious and time-consuming.

Cold-capture is a technique that allows labelling of antibody producing cells with a fluorescent reagent that binds to the antibody molecules when they are transiently present on the cell membrane before release from secretory vesicles. This labelling could enable cell sorting based on the level of fluorescence signal to select higher productivity cells. In this study, the featured speaker sought to test the ability to enrich high titer IgG CHO cell clones by cold-capture, followed by isolation of single cells with the brightest fluorescent signal using the Namocell Pala Single Cell Dispenser — a fully automated dispenser that enables a fast, easy fluorescence and light scatter-based single cell isolation. The speaker will show that their method allowed the identification of clones with an average 3x higher IgG production from the cold-capture labelled cells compared to non-cold-captured cells.

These results suggest that the combination of cold-capture and single cell isolation could be an efficient and economical solution for improving cell line development in recombinant antibody production.

Join this webinar to learn more about the potential of cold-capture-facilitated single cell isolation and cloning to improve the efficiency in identifying high yield clones.

Speaker

Francisco Ramirez, Manager, Application Science, Bio-Techne

Francisco Ramirez is a biotech industry professional with over 23 years of experience. He received his BS in biological sciences from the University of California, Davis. He has worked at ProteinSimple, a Bio-Techne brand, for more than 14 years, primarily as a Research Scientist in the R&D and Applications Science groups. His expertise in assay and product development led to the development of key reagents for the Simple Western and Maurice capillary electrophoresis platforms. Joining the Applications Science team in 2017, he has led the development of many important applications published in application and technical notes, protocols and scientific posters. He is currently the Manager of Applications Science, leading a team focused on the Simple Western and Namocell platforms.

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