Harnessing mRNA Immunization Strategy for Challenging GPCR Protein Antibody Discovery

Life Sciences, Drug Discovery & Development, Fundamental Research,
  • Friday, May 10, 2024

Multi-transmembrane proteins, especially G-protein coupled receptors (GPCRs), are extremely difficult to develop antibodies against due to their low expression levels, insolubility in vitro, unstable purification and difficulty in maintaining their native conformation, which has greatly hindered the development of antibody drugs against GPCRs.

In this webinar, the speaker will discuss the use of mRNA as a brand-new antigen format for antibody development targeting transmembrane proteins. The mRNA-expressing target protein could be delivered in vivo through lipid nanoparticles (LNP), where the translated antigen directly stimulates the immune response in the host.

In this way, complex and tedious antigen extraction and purification steps in vitro could be completely avoided, greatly simplifying the GPCRs’ antibody production process. Next, mRNA immunization could be combined with traditional hybridoma fusion, B cell cloning or Beacon single B cell sorting platform for positive B cell screening to develop mouse/rabbit monoclonal antibodies.

R&D and production data showed that multiple transmembrane proteins could be custom-designed into mRNA for successful immunization with inspiring serum titer after two doses. In comparison with the traditional immunization strategy, the monoclonal antibody developed from mRNA-LNP immunization exhibited higher affinity and probability of recognizing/binding to natural antigens than protein, DNA or cell line immunization. Furthermore, mRNA-LNP immunization for antibody production could also be applied to other types of proteins, including secreted proteins, bacterial proteins and protein fragments.

Register for this webinar today to understand how an mRNA immunization strategy opens a new avenue in the antibody production of difficult protein targets and accelerates the development of antibody drugs against difficult-to-express targets.


Dr. Tingting Xiao, Antibody Senior Scientist, GenScript

Tingting Xiao graduated with a Ph.D. degree from the University of Hong Kong in August 2022. She has authored four impactful papers in esteemed SCI journals, with three securing a coveted Q1 ranking and one in Q2, all under her leadership as the first author. Dr. Xiao joined GenScript as Senior Scientist in October 2022, and is currently responsible for the development of multiple transmembrane protein antibody development projects and the optimization of antibody sequencing platform. She has now delivered multiple antibodies to membrane proteins, applying mRNA immunization platform. Dr. Xiao’s innovative contributions exemplify her commitment to scientific advancement and position her as an important figure in antibody development.

Message Presenter

Who Should Attend?

This webinar will appeal to academic and industry professionals in the following fields:

  • Medical immunology
  • Molecular biology
  • Vaccine development
  • Antibody discovery and engineering
  • GPCR antibody drug development

What You Will Learn

Attendees will learn about:

  • The background of GPCRs’ antibody development
  • Strategies in GPCRs’ antibody discovery
  • Overcoming challenges in transmembrane proteins’ antibody development via GenScript’s mRNA immunization strategy

Xtalks Partner


GenScript is the leading contract research organization in the world providing gene, peptide, protein, CRISPR, and antibodies. Since its foundation in 2002, GenScript has grown exponentially through partnerships with scientists conducting fundamental life science research, translational biomedical research, and early-stage pharmaceutical development. The company is recognized as having built a best-in-class capacity and capability for biological research services, encompassing gene synthesis, peptide synthesis, custom antibody and protein engineering, and in vitro and in vivo pharmacology – all with the goal to Make Research Easy.

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