Sequencing Cells without Killing Them Using Subcellular Biopsies

Life Sciences, Pharmaceutical, Drug Discovery & Development, Fundamental Research,
  • Wednesday, October 09, 2024 | 9am EDT (NA) / 2pm BST (UK) / 3pm CEST (EU-Central)
  • 60 min

Discover how live-seq can capture the molecular dynamics of a single cell and provide temporal readouts of cellular state transitions.

Sequencing of thousands of cells can reveal the intricacy and abundance of different cell types and states within a population. However, when inferring cell trajectories, the fundamental conundrum of not being able to measure the same cell along its trajectory needs to be overcome.

As such, the dynamics of underlying biological processes must be assumed from static snapshots of different cells at different stages. As a result, differential expression patterns of minor cell populations might be missed, which can become problematic when few cells within a population change their fate and dedifferentiate into a cancerous or metastatic state.

In this webinar, live-cell sequencing (Live-seq), a new approach to single cells, will be discussed. Live-seq utilizes subcellular biopsies to record the transcriptome of a cell while keeping it alive.

During this webinar, the expert speaker will take you along to explain how Live-seq works with a special focus on how the FluidFM OMNIUM platform facilitates the single-cell biopsy workflow. A comprehensive demonstration of the experiment will also be presented.

Register for this webinar today to gain insights into the use of subcellular biopsies for cell sequencing and how live-seq can capture the molecular dynamics of a single cell.

Speaker

Tamás Gerecsei, Cytosurge

Tamás Gerecsei, FluidFM Expert, Cytosurge

Tamás Gerecsei holds a PhD from Eötvös Loránd University, Budapest, where he worked with the FluidFM technology to study cell adhesion. After completion of his PhD, he joined Cytosurge to help push the limits of the technology and make it available to researchers around the world.

Message Presenter

Who Should Attend?

This webinar will appeal to:

  • Researcher
  • Post-doc
  • Associate or Assistant Professor
  • Professor
  • Technician

What You Will Learn

Attendees will learn about:

  • How 1-2 pL cytoplasmic biopsies are collected from HeLa and Panc1 cells on the FluidFM OMNIUM platform while keeping them alive
  • How cells are tracked inside the platform to enable sampling of the same cell 24 h later
  • How cytoplasmic biopsies from Panc-1 cells can be analyzed using LuthorHD to record single-cell transcriptomes

Xtalks Partner

Cytosurge

Cytosurge AG is a biotechnology company that develops innovative research solutions based on the FluidFM ® technology for high-precision single-cell manipulation. FluidFM combines microfluidics and force microscopy, which enables gentle interactions with cells. With the capabilities of FluidFM, we seek to advance the comprehension of intricate cellular processes, thus fostering new dimensions in cellular analysis.

The single-cell biopsy solution on the FluidFM OMNIUM platform provides a unique workflow for extraction of a part of the cytoplasm from individual living cells while preserving their viability. These cytoplasmic biopsies can be used for subsequent highly sensitive, low-input RNA-seq analysis to characterize single-cells multiple times throughout their lifetime.

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