Poseida is seeking outstanding candidates to join our Genetic Engineering team focusing on molecular biology, protein engineering, and gene editing. They will play a key role in exciting projects focused on expanding and evolving the use and capabilities of our piggyBac gene delivery and Cas-CLOVER gene editing platforms.

Responsibilities 

These may include but are not limited to:

• Expand our proprietary gene editing platform to develop new tools and/or applications for specialized and enhanced implementation in gene therapy, gene editing, and biologics/cell therapeutics
• Apply your strong background in molecular and cellular biology to develop and/or enhance new gene editing and transposon tools in cell culture models for use in ex vivo and in vivo therapies
• Work in a highly collaborative, cross-functional team environment to perform experiments in the lab, analyze results, and communicate findings in team meetings
• Keep current on trends, new products and technologies in gene editing, knock-ins, and transposons
• Actively participate in our proprietary gene therapy platform technologies to create new human therapeutic candidates
• Analyze data from cell-based assays and report to multidisciplinary groups
• A Sr. Research Scientist may manage 1-2 Research Technicians or Research Associates so would expect management experience as well as 5+ years relevant industry experience, at that level.

Requirements, Knowledge, Skills and Abilities

• Ph.D. in molecular biology, synthetic biology, cell biology, virology, protein engineering, genome engineering, or related scientific field with 0-2 years at the Research Scientist I level, 3-5 years at the Research Scientist II level and 5+ years of relevant industry experience at the Sr. Research Scientist level. An equivalent combination of education and experience may be considered. The title and responsibility commensurate with relevant experience.
• Experience with transposases, recombinases, CRISPR, TALENs, ZFNs, Prime Editing, Base Editing, or other genome editing tools
• Proficiency in bioinformatics/machine learning/computer languages (such as Python) highly valued
• Previous experience with functional assays and analyses used to measure success of gene editing/knock-in/knock-outs and genotoxicity, including insertion site characterization, off-target evaluation, and genomic instability assessment
• Understanding and hands on experience with various directed evolution or rational/structure-based protein engineering strategies is a plus
• Experience with cloning techniques such as restriction digests, Gibson assembly, transformations, Sanger sequencing, plasmid preps, and DNA design/analysis software. High throughput or pooled cloning work is a plus
• Proficiency with mammalian tissue culture and transfection of immortalized cell lines. Experience with various primary cell types is a plus
• Previous experience or a strong desire to learn the following techniques:
•       Multicolor flow cytometry, cell sorting, and FACS analysis software
•       PCR, qRT-PCR, ddPCR, in vitro transcription and RNA preparation
•       Library preparation and Next Generation Sequencing on Illumina and nanopore platforms
•       Lentiviral production and transduction
•       Protein purification, Western Blot, chromatography
• Demonstrated ability to be independent, self-motivated, as well as work in a highly collaborative setting
• Excellent oral and written communication skills, attention to detail, and documentation skills
• Excellent critical thinking and analytic skills and great trouble-shooting ability are required
• Must be passionate about performing research that may lead to the treatment or cure of human disease
• Able to lift to 40 pounds and stand for hours at a time
• The position requires a combination of standing or sitting in a laboratory environment as well as walking around the facility