Cell-free systems aim to capture some properties and functions of living cells without using living cells. By building cell-free systems such as artificial cells and cell-free metabolic pathways, one can try to understand how cells work and/or exploit unique functions of cells without complexities associated with using living cells. In this webinar, we describe cell-free “riboswitches” as molecular devices that enable cell-free systems to sense chemical signals and respond by regulating protein production.
In this webinar, the featured speaker will discuss the semi-rational design of histamine-responsive riboswitches in a cell-free translation system and in liposome-based artificial cells. Furthermore, a high-throughput approach to cell-free riboswitch development aided by microfluidic droplet sorting that drastically streamlines the design process will be discussed. Lastly, the featured speaker will discuss how cell-free riboswitches were used to demonstrate “intercellular” communication among droplet-based artificial cells.
Join this webinar to learn about cell-free riboswitches, their development using microfluid droplet sorting and their role in intercellular communication.
Speaker
Yohei Yokobayashi, PhD, Professor, Nucleic Acid Chemistry and Engineering Unit, Okinawa Institute of Science and Technology
Yohei Yokobayashi is a Professor at the Okinawa Institute of Science and Technology Graduate University (OIST).
After studying organometallic chemistry and peptide chemistry at the University of Tokyo (B.Eng. and M.Eng.), he received his Ph.D. from The Scripps Research Institute in 2001. After working as a postdoc at Caltech with Prof. Frances Arnold, he joined the Department of Biomedical Engineering at the University of California, Davis as an Assistant Professor (2004-2010) and was promoted to an Associate Professor (2010-2015). Now he leads the Nucleic Acid Chemistry and Engineering Unit at OIST which focuses on chemical biology and synthetic biology of nucleic acids.
Message PresenterWho Should Attend?
This webinar will appeal to researchers interested in:
- Synthetic biology
- Cell-free protein synthesis
- Engineered nucleic acid
- Riboswitches
- Aptamer and droplet microfluidics
What You Will Learn
Attendees will gain insights into:
- How cell-free systems can capture some cellular functions without biological constraints and complexities
- How cell-free riboswitches enable cell-free systems to sense and respond to chemical signals
- The use of droplet sorting as a powerful tool for designing molecular devices that functions in cell-free systems
Xtalks Partner
On-chip
On-chip Biotechnologies Co., Ltd, Tokyo, Japan, is the first company in the world to launch a microfluidic chip-based cell sorter on the market. On-chip Biotechnologies offers four innovative products, On-chip Sort (microfluidic chip cell sorter), On-chip Droplet Generator (emulsion droplet generator), On-chip SPiS (single cell/particle dispenser) and On-chip Droplet Selector (droplet sorter and dispenser). On-chip Sort was developed to allow damage-free, contamination-free and sterile cell sorting, which are difficult to attain with conventional sorters. On-chip Sort allows sorting of cells, particles, emulsion droplets and gel balls sized up to 130 µm, in any sheath fluid of the user’s choice. The other three instruments were developed to complement On-chip Sort. On-chip Droplet Generator produces stable and monodisperse water-in-oil emulsion droplets. On-chip SPiS allows accurate dispensing of individual cells and particles sized up to 200 µm into well plates. On-chip Droplet Selector is capable of sorting and dispensing of emulsion droplets. On-chip strives to assist researchers in accelerating the world’s new discoveries in life science, drug development and diagnostics with our Japanese innovative microfluidic technologies.
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