The clinical manifestations of COVID-19 infections are highly variable between individuals and are not localized to the lungs and airway passages as seen with other respiratory viruses. In order to define appropriate therapeutic targets and mitigation strategies for COVID-19 infections, it is important to understand the impact of COVID-19 across a wide variety of organs at the cellular level.
To that end, we have conducted a survey of the effects of SARS-COV-2 infection on host gene expression, at the single cell level, across a wide array of tissues obtained from autopsies of confirmed COVID-19 patients. Tissues from nineteen COVID-19 autopsy patients, including seven from the US and twelve from Europe, were analyzed using single nuclei RNA sequencing (snRNA Seq), along with three non-COVID controls.
Six to eight different organs from each patient were analyzed, including heart, lung, liver, kidney, ileum, colon, spleen, prostate and testes. In all, gene expression analyses were performed on approximately 150 unique tissue specimens. The project required implementation of new experimental workflows and technologies to enable safe handling of the samples.
Register for this webinar to hear a presentation of these methods along with the results detailing the impact of COVID-19 on host gene expression.
Bruce Wang, Assistant Professor in Residence, Div. of Gastroenterology, UCSF
Bruce was born in China and grew up in the San Francisco Bay Area. He went to college at Stanford and did his medical school, residency and fellowship at UCSF. After a postdoctoral fellowship at Stanford, he came back to UCSF to start his lab. Dr. Wang studies how the different cell types in the liver, in particular hepatocytes, are generated during development, patterned and maintained during adulthood, and regenerate after injury. His long-term goals are to improve the understanding of liver disease pathophysiology and develop novel methods of treatment for liver diseases, including cell replacement therapy. Currently, he has two major research focuses: 1) understanding the biology of adult hepatocyte stem cells and 2) developing a liver cell atlas. His lab takes innovative and integrated approaches to address these two areas using the tools of stem cell biology, developmental biology, genomics and tissue engineering.
Who Should Attend?
This webinar will be relevant to scientists researching genetic changes in tissue as a result of infection, including those in academia, government, hospitals and industry with relevant job titles including:
- Research Scientist
- Postdoctoral Fellow
- Genome Core Facility Director
- Single Cell Sequencing Core Facility Director
- Senior Scientist, Functional Genomics
- Senior Scientist, Infections Diseases
- Director of the Office of Genomics
- Director of Virology Research Centers
What You Will Learn
- New experimental workflows and technologies to enable safe handling of tissue from virally infected patients
- The benefit of automated tissue dissociation into nuclei for single cell sequencing applications, as demonstrated on heart, lung, liver, kidney, ileum, colon, spleen, prostate and testes human samples
- The impact of COVID-19 in host gene transcription at single cell resolution
- The impact of COVID-19 on host gene expression in a wide variety of organs
S2 Genomics has automated and integrated the processes required to dissociate solid tissue into suspensions of single cells or nuclei. The Singulator 100 system allows scientists to perform reproducible analyses of solid tissue samples easily and routinely for single-cell genomics and cell biology applications