Is Hybrid LC-MS/MS Becoming the New Gold Standard?

  • Friday, April 22, 2022

Due to the relative maturity of the technology, most people’s instincts when thinking about bioanalysis of large molecules is towards ligand binding assays. In this webinar, the featured speakers urge people to be open to a more flexible approach — Hybrid LC-MS/MS.

Hybrid liquid chromatography with tandem mass spectrometry (LC-MS/MS) can be a useful tool for quantitation of biological therapeutics and biomarkers. Here, the principles of ligand binding are combined for extraction, enzymatic digestion and the powerful downstream selectivity of LC-MS/MS. Moreover, flexibility in surrogate peptide selection can be used to provide much more information about different regions of the molecule. This combined with less need for “great” antibodies can help drive the drug development process.

Join this webinar to learn about the many applications of Hybrid LC-MS/MS and see how it can offer additional information to supplement analyte quantitation.


John Perkins, KCAS Bioanalytical and Biomarker Services

John Perkins, Senior Scientific Advisor, KCAS Bioanalytical and Biomarker Services

John R. Perkins is the Senior Scientific Advisor with a focus on LC/MS technologies at KCAS. Previously, he was at Q2Solutions and legacy companies for over 24 years working in quantitative LC/MS, principally with small molecules. His primary focus was on validation and sample analysis processes as well as managing customer relationships in Ithaca, NY. More recently, he was responsible for the bioanalytical lab in Oss, The Netherlands.

John received his PhD in supercritical fluid chromatography/mass spectrometry from University of Wales, College of Cardiff, UK. He did postdoctoral research on nanoscale separation techniques with mass spectrometry at NIEHS in Research Triangle Park. He earned his bachelor’s degree in chemistry from University of Reading, UK and a master’s degree in advanced analytical chemistry from University of Bristol, UK.

Message Presenter
Dawn Dufield, KCAS Bioanalytical and Biomarker Services

Dawn Dufield, Senior Director, Biopharmaceutical LC-MS/MS Services, KCAS Bioanalytical and Biomarker Services

Dawn R. Dufield is the Senior Director of Biopharmaceuticals (LC-MS/MS) Services at KCAS. Previously she had been with Pfizer and legacy companies for over 20 years working in the quantitative LC-MS/MS field. She was one of the early pioneers of using immunoaffinity combined with LC-MS/MS to offer additional selectivity which is now commonly referred to as Hybrid LCMS. She is currently a member of the AAPS Mass Spec Protein Bioanalysis Committee (MSPBC) and an active member of the American Society for Mass Spectrometry (ASMS). She has numerous publications and presentations in her field and recently contributed to a white paper on recommendations for validation of LCMS-based bioanalytical methods for protein biotherapeutics.

Dawn received her PhD in bioanalytical chemistry (honors) from the University of Kansas in Lawrence, Kansas. She earned her bachelor’s degree in chemistry (computer minor) from Southwest Missouri State University in Springfield, MO.

Message Presenter

Who Should Attend?

  • Pharma/Biopharma
  • CEO
  • Directors
  • Managers

What You Will Learn

Attendees will learn:

  • Why might there be a need for Hybrid LC-MS/MS in large molecule quantitation
  • Principles of Hybrid LC-MS/MS
  • Advantages of Hybrid LC-MS/MS
  • Case studies illustrating the value of Hybrid LC-MS/MS

Xtalks Partner

KCAS Bioanalytical and Biomarker Services

KCAS Bioanalytical and Biomarker Services, an Integris BioServices, LLC Company is a rapidly growing company that provides comprehensive GLP compliant development services from early discovery support through product registration and beyond. One of only a select few CROs offering both large and small molecule development with extensive capabilities and expertise, KCAS provides a truly differentiated approach to its clients who are looking for both scientific expertise and excellent customer service. KCAS is headquartered in the Kansas City area, with additional locations worldwide.

A Few Quick Facts About KCAS

  • Ranked in top 50 fastest growing companies in 2018 by Kansas City Business Journal
  • KCAS received the 2018 Good Business Award from Shawnee Chamber of Commerce
  • Named in the Kansas City Business Journal as one of the Top Animal Health Companies
  • Named Best Companies to Work for by Ingram’s Magazine in 2016
  • Received 2016 Business Excellence Award from Kansas Department of Commerce
  • Headcount more than doubled from 2015 to 2018 (many recruited outside KS)

Further information regarding KCAS can be found at

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