RNA and DNA therapeutic molecules have rigorous requirements for purity. Oligonucleotide purification is frequently done using high resolution anion exchange chromatography (AIEX) to separate erroneous or incomplete sequences from full-length sequences.
In this webinar, the featured speaker will discuss strategies for oligonucleotide purification. Three case studies will be shown about the effects of temperature, residence time and resin type on the loading capacities of pegylated RNA, 45-nucleotide-long RNA and 20-mer DNA. The featured speaker will present the analysis of the correlation between yield and purity under optimal conditions. These optimized conditions resulted in DNA and RNA products with high purity and yield.
Speaker
Cecilia Unoson, Manager of Applications, Bio-Works
Cecilia Unoson is Manager for the Applications Research team at Bio-Works. She joined the company in the beginning of 2018. She is a molecular biologist with a PhD in microbiology (Uppsala University). Prior to her work here she has worked in both smaller companies (Olink) as well as in bigger companies (Cytiva) and also spent many years as PhD student, post doc and researcher in academia studying regulatory RNAs in bacteria, especially focusing on the Crispr-Cas9 system.
Message PresenterWho Should Attend?
This webinar will benefit professionals working on chromatography and downstream process development, with relevant job areas that include:
- Process developers focused on the production of therapeutic oligonucleotides
- Product developers focused on therapeutic oligonucleotides
- Scientists involved with oligonucleotide purification
- Scientists, molecular biology
- Analytical chemists
What You Will Learn
Join for this webinar to learn:
- The standards for oligonucleotide separation based on the phosphate backbones which are negatively charged
- The optimization process, including the important parameters and how these parameters could be different for different molecules
- How yield and purity can be optimized and how to understand their correlation when purifying DNA and RNA
- The significance of good resin choice with optimized properties regarding pore size and bead size
Xtalks Partner
Bio-Works
Bio-Works is based in Uppsala, Sweden. We develop, produce and deliver innovative agarose-based products for purification of peptides, proteins and other biomolecules to industry and research organizations in the Life Science field. The employees in the company have long experience from the biotechnology industry and deep knowledge about the development of high-performance purification products. This has made it possible to develop a broad and high-performance product portfolio available in various prepacked formats as well as in several different bulk sizes.
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