Success Stories for Challenging pDNA and Viral Vectors Downstream Processing

Join this webinar to learn how target-specific adsorbents enable increase in productivity due to increased manufacturing speed and recovery yield while achieving the purity levels for the product quality attributes required by regulatory authorities.

A novel purification approach is required to address the complex purification challenges of critical raw materials for cell and gene therapies (CGT) such as plasmid DNA (pDNA) and lentiviral vectors (LVV). The purification technology needs to be optimized for large and fragile CGT modalities, allowing downstream processing under low operational pressure and low salt buffers.

In this webinar, the expert speakers will share data and insights on the development of a low salt, high recovery yield and lentiviral vector purification workflow, with efficient removal of impurities such as host cell proteins and dsDNA. Attendees will also learn how the performance of the anion exchange adsorbent compares to alternative commercially available chromatography options. The expert speakers will also discuss a scalable downstream workflow for efficient pDNA manufacturing at full-speed operation while maintaining low column pressure.

Attendees will also learn how processing of highly viscous feeds such as large pDNA sequences (e.g., greater than 10 kb) required in CGT can be considerably simplified. The speaker will also demonstrate how the hydrophobic adsorbent integrated into the flexible workflow can be used as a capture step or a polish step to adapt to the varying and growing process needs.

Register for this webinar today to gain insights into increasing the downstream processing and purification of cell and gene therapeutics.

Speakers

Ian Scanlon, Nanofiber Development Lead, Astrea Bioseparations

Ian Scanlon has 25 years’ experience in drug discovery and reagent development focused on scale-up and purification working at Roche, CRUK, UCB, Illumina and Sigma Aldrich. For the past decade, Ian has worked on convective adsorbent development and process design for biologics. Currently, Ian leads the team developing nanofiber adsorbent solutions for ATMP purification at Astrea Bioseparations in Cambridge, UK.

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Dr. Christie Childers, Senior Scientist, Downstream Processing, Astrea Bioseparations

Dr. Christie Childers is a Senior Scientist at Astrea Bioseparations leading the next-generation development of nucleotide purification solutions based on novel nanofiber technology. Her journey began in the field of molecular biology at Carleton University in Ottawa, Canada, where she received a PhD investigating enzyme purification and post-translational modification for metabolic regulation.

Christie now has four years’ industry experience in downstream process development with a past focus on customer-based R&D, process scale up and technical transfer of nucleotide workflows at CPI. Christie now leads and develops nanofiber-based pDNA and mRNA processes at Astrea Bioseparations.

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