Purification of Oligonucleotides Using Anion-Exchange Chromatography

Therapeutic RNA and DNA molecules have a stringent requirement for purity, often achieved using high-resolution anion-exchange chromatography which separates full-length sequences from incomplete or erroneous sequences.

In this webinar, our featured speaker will discuss the common purification strategies for oligonucleotides. Three case studies will be presented on the effects of residence time, temperature and type of resin on loading capacities for a 20-mer DNA, a 45 nt-long RNA and a pegylated RNA. The expert speaker will also discuss an analysis of the correlation between purity and yield using optimized conditions. These conditions generated DNA and RNA products with high yield and purity.

Key topics of discussion will include:

• The principles for separation of oligonucleotides based on their negatively-charged phosphate backbones
• Which parameters are important in the optimization process and how these parameters may differ for different molecules
• How to optimize yield and purity and to understand their correlation in DNA and RNA purifications
• The importance of choosing a good resin with optimized properties in regard to bead size and pore size

Register for this webinar to learn about best practices for using anion-exchange chromatography to purify therapeutic oligonucleotides.

Speaker

Cecilia Unoson, Manager — Applications, Bio-Works

Cecilia Unoson is Manager for the Applications Research team at Bio-Works. She joined the company in the beginning of 2018. She is a molecular biologist with a PhD in microbiology (Uppsala University). Prior to her current position, she has worked in both smaller companies (Olink) as well as in bigger companies (Cytiva) and also spent many years as PhD student, post doc and researcher in academia studying regulatory RNAs in bacteria, especially focusing on the CRISPR-Cas9 system.

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