Clustered regularly interspaced short palindromic repeats (CRISPR) technology is now widely utilized in fields such as gene and cell therapy, animal and cell model development and genetic engineering. The rational design of single guide RNA (sgRNA) and homology-directed pair (HDR) template sequences is the most crucial step for the success of these experiments.
In this webinar, the expert speaker will share her extensive experience with CRISPR projects and discuss key insights for successful CRISPR experiments. Topics will include:
- Designing highly specific sgRNAs with minimal off-target effects
- Optimizing HDR templates to enhance knock-in efficiency
- Practical demonstrations of sgRNA and HDR designs using GenScript’s tools for gene knockout, point mutation, small and large insertions and large fragment deletions
- How to leverage GenScript’s free GenCRISPR sgRNA and HDR design tools to simplify the design process and improve editing efficiency
This webinar will thoroughly explore the intricacies of designing sgRNA and HDR templates for CRISPR gene editing, emphasizing high editing efficiency and minimizing off-target effects. The attendees will also learn the essential processes and skills required to achieve these objectives. The speaker will also discuss how to select the most appropriate sequences for different applications after considering specific parameters that influence the outcome.
Moreover, the session will include case studies covering various design scenarios, such as gene knockout, point mutations, small and large insertions and large fragment deletions, thus providing practical insights and examples. This comprehensive coverage will equip participants with the knowledge and tools necessary to execute precise and efficient gene editing techniques.
Register for this webinar today to gain expert insights into highly specific sgRNAs, enhancing knock-in efficiency with optimized HDR templates and optimizing CRISPR editing efficiency.
Speaker
Jie Zhu, PhD, Field Application Scientist – Life Science Group, GenScript Biotech Corporation
Dr. Jie Zhu holds a PhD in Developmental Genetics from Miami University and is an expert in stem cells and regenerative biology, focusing on the development of innovative regenerative therapies for neurodegenerative diseases. Her expertise spans disease modeling, genome engineering, and the advancement of gene and cell therapies.
As an Application Scientist, she has specialized in the application of CRISPR technology in gene and cell therapy development, playing a crucial role in supporting numerous researchers with CRISPR gene editing designs and providing technical guidance, particularly in areas like CAR-T cell engineering.
Additionally, Dr. Zhu developed the GenCRISPR sgRNA design tool and HDR template design tool, both of which are grounded in her extensive knowledge and experience and have been validated to demonstrate excellent editing efficiency.
Message PresenterWho Should Attend?
This webinar will appeal to academic researchers and industry professionals interested in CRISPR-related applications:
- Students and research fellows planning to use CRISPR genome editing technology in their research projects
- Academic researchers and Laboratory Directors in the field of CRISPR research
- Research Scientists/Senior Scientists/Project Leaders from R&D departments within biotechnology or biopharmaceutical organizations
What You Will Learn
Attendees will learn about:
- The process and skills needed to design sgRNA and HDR templates with high editing efficiency and low off-target effects
- How to select appropriate sequences for different applications based on specific parameters
- Case studies on designs for gene knockout, point mutations, small and large insertions and large fragment deletions
- The features and advantages of the GenCRISPR sgRNA and HDR design tools
Xtalks Partner
GenScript
GenScript is the leading contract research organization in the world providing gene, peptide, protein, CRISPR, and antibody. Since its foundation in 2002, GenScript has grown exponentially through partnerships with scientists conducting fundamental life science research, translational biomedical research, and early stage pharmaceutical development. The company is recognized as having built a best-in-class capacity and capability for biological research services, encompassing gene synthesis, peptide synthesis, custom antibody and protein engineering, and in vitro and in vivo pharmacology – all with the goal to Make Research Easy.
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