Library sizes of >1012 enzyme variants can be generated during enzyme engineering. However, conventional microtiter plate- or agar plate-based screening systems only allow a few thousand variants to be screened, leaving most of the generated variants unexplored. Therefore, ultrahigh-throughput screening (uHTS) technologies are critical for exploring the diversity of enzymes in a library.
In uHTS, a link must be made between each signal and the activity of the enzyme variant, and between the genotype and the generated signal (phenotype). Samplix’s two sizes of double-emulsion droplets, designated DE20 and DE50, form a closed compartment for cell growth and assays. This provides the link between genotype and phenotype.
Moreover, cells producing rare, active enzyme variants are often outgrown in bulk cultivation, greatly complicating the identification of potentially improved variants. Encapsulated cells grow in isolation, so rare, active variant-producing cells are not out-competed.
Samplix has developed a workflow using Xdrop and the Xdrop DE20 Cartridge to encapsulate living bacterial cells producing an enzyme library with rare, active variants in DE20 droplets. The droplets support:
- Fluorescence-based analysis in a unique single-cell format and sorting using a cell sorter or Xdrop Sort
- Recovery of the cells producing the desired variants for further growth and analysis
Register for this webinar to learn how Xdrop improves the efficiency of enzyme engineering by increasing the throughput for fluorescence-based screening.
Volkan Besirlioglu, Field Application Scientist, Samplix ApS
Volkan Besirlioglu received his BSc in Bio- and Nanotechnologies from the University of South Westphalia and his MSc in Molecular and Applied Biotechnology from RWTH Aachen University. At the same university, he then conducted research in the group of Professor Ulrich Schwaneberg, developing ultra-high throughput screening systems based on biomimetic compartments for protein engineering and metagenomics.
Who Should Attend?
This webinar will benefit researchers, heads of research, CTOs and other decision makers at corporate and academic facilities involved in:
- The engineering of enzymes and other proteins
- The screening of cells to look at antibody libraries
- The screening of microbial and mammalian cells using flow cytometry
- The development of microbial food and feed ingredients
The presentation can also be of interest laboratory technicians and professionals involved in expanding the library of flow cytometry assays at their facility.
What You Will Learn
Attendees will learn about:
- The limitations of conventional enzyme screening and the need for uHTS in enzyme engineering
- Advancements in enzyme variant selection and analysis with Samplix’s Xdrop technology
- Improving enzyme engineering efficiency by increasing the throughput of fluorescence-based screening with Xdrop
Samplix ApS supports the life sciences and medical research communities with microfluidics-based solutions designed to deliver high-resolution insights into cells and genomes. Samplix stands ready to help academic, corporate and government researchers reach their goals in areas as diverse as engineered cell therapy, molecular engineering, and cell biology. We have developed unique microfluidics instruments to support molecular engineering: Xdrop and Xdrop Sort. These instruments enable high-throughput, sensitive, and accurate screening or functional population analysis with single-cell resolution by encapsulating living cells in highly stable double-emulsion droplets. These picoliter-sized droplets act as microenvironments for incubation and screening. Xdrop can change how you analyze and work with cells.