mRNA has emerged as a powerful tool across vaccines, protein replacement therapies and gene editing. Central to these innovations is the need for ultra-fast, high-quality in vitro transcription (IVT) templates. Yet, researchers often face challenges in template preparation, from poly(A) tail instability, suboptimal template formats and designs to impurities that compromise mRNA quality and downstream applications.
In this webinar, the featured speaker will outline a comprehensive strategy for designing and preparing IVT-ready DNA templates, covering:
- Vector and backbone selection to support cloning, stable propagation and efficient linearization
- UTR and poly(A) tail design, including segmented poly(A) strategies that improve plasmid stability without sacrificing translational performance
- Comparison of IVT template formats, from plasmid-based linear templates to emerging cell-free synthesis methods
- Critical quality control parameters, including verification of linearization, removal of host-derived contaminants and purity thresholds that directly affect IVT outcomes
- Future directions in IVT template development, with a focus on scalable, automated and cell-free workflows that accelerate research timelines
In addition, the featured speaker will introduce GenScript’s latest innovation, the Cell-Free IVT Template service, which delivers IVT-ready DNA starting from gene synthesis in as fast as six business days. This platform combines speed, purity and consistency to support efficient IVT and reliable mRNA output.
Whether focused on early discovery, high-throughput screening or translational research, this session will provide practical guidance to streamline mRNA workflows.
Register now to learn how integrated solutions — from vector design to cell-free IVT template preparation — can streamline the production of high-quality mRNA templates and accelerate research outcomes.
Speaker
Andrew Tsao, Global Product Manager, GenScript
Andrew Tsao is the Global Product Manager at GenScript, covering the Molecular Biology portfolio. His focus includes plasmid solutions for key applications such as mRNA, AAV, LVV as well as the development of innovative technologies that support cutting-edge research in gene and cell therapy, as well as synthetic biology.
Message PresenterWho Should Attend?
This webinar will appeal to:
- Biotech and pharma professionals developing mRNA-based vaccines, therapeutics or gene editing applications
- Researchers and scientists working on mRNA design, IVT optimization or therapeutic development
- Molecular biology and RNA production teams seeking to improve IVT template quality and reproducibility
- Teams looking for faster, more scalable IVT template solutions to accelerate early-stage or preclinical workflows
What You Will Learn
Attendees will:
- Understand the critical role of poly(A) tail integrity in IVT yield, mRNA stability and translational efficiency
- Learn how vector backbone design and poly(A) strategies influence plasmid propagation and mRNA performance
- Compare different IVT template formats, including linearized plasmids and emerging cell-free synthesis methods
- Discover essential quality control parameters, such as linearization, residual host DNA/protein removal and purity thresholds, that affect IVT success
- Explore how GenScript’s end-to-end solution, including engineered backbones and the new Cell-Free IVT Template service, accelerates template generation with high quality and fast turnaround
Xtalks Partner
GenScript
GenScript is the leading contract research organization in the world providing gene, peptide, protein, CRISPR, and antibody. Since its foundation in 2002, GenScript has grown exponentially through partnerships with scientists conducting fundamental life science research, translational biomedical research, and early stage pharmaceutical development. The company is recognized as having built a best-in-class capacity and capability for biological research services, encompassing gene synthesis, peptide synthesis, custom antibody and protein engineering, and in vitro and in vivo pharmacology – all with the goal to Make Research Easy.
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