Size Exclusion Chromatography: How to Select an SEC Column and Optimize a Method

Life Sciences, Drug Discovery & Development, Laboratory Technology,
  • Wednesday, December 11, 2019

Biotherapeutic development is a critical component of drug manufacturer pipelines as the pharmaceutical industry moves toward more targeted therapies. Liquid chromatography plays an important role in biotherapeutic development as it assists in identity and purity assessments. Aggregation of protein-based therapeutics is the most common critical quality attribute (CQA) analyzed through the drug development process. Because aggregation is so important, there is an abundance of size exclusion chromatography (SEC) options on the market that can determine levels of aggregation of a protein biotherapeutic. Because each SEC column possesses unique properties, methods must be adjusted to match the column choice. This free webinar will cover the basics of size exclusion chromatography, how to select an SEC column and method development considerations for protein aggregate analysis.



Brian Rivera, Senior Global Product Manager, Biologics, Phenomenex Inc.

Brian Rivera has worked at Phenomenex for 9 years, holding other positions including technical support and sales. Prior to joining Phenomenex, Brian worked within the biotechnology industry, including positions focused on protein purification, analytical methods development and in-process analytical support. Brian has a Bachelor’s degree from the University of California, Davis.

Message Presenter

Who Should Attend?

This webinar will appeal to all scientists involved in biotherapeutic development including: 

  • Expert analytical R&D scientists
  • Entry-level scientists in pharmaceutical or life sciences companies
  • Scientists from academia

What You Will Learn

Attendees will hear about: 

  • An overview of biotherapeutic aggregation and size exclusion chromatography
  • The role of chromatography hardware in aggregate analysis
  • How method parameters such as pH, flow-rate and salt concentration influence SEC
  • Adaptation of SEC methods to mass spectrometry through native mass spectrometry (MS) method development

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Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, clinical research, government and academic laboratories. Phenomenex chromatography solutions accelerate science and help researchers improve global health and well-being.

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